Suite GHJ Trivia

Upstream

100

What is the antibiotic used in Fermentation?

Kanamycin

200

What grade is the BSC found in the inocc room

B

300

How many filter integrity tests do we perform in GH (no backup filters)

6 (Ferm F1, F2. F4, F5, F7. FF)

400

Name all reagents added to the flask in seed lab

kanamycin

vitamin B 

dextrose

plasmid seed media 

E. Coli cell  

500

What is the target harvest criteria for Inocc and Ferm

3.00 - 5.00 OD 

90 +/- 10 OD and 31 +/- 2hours  

Downstream

100

How many total usages can be used in GH for resin and membranes 

20

200

What is the max volume of TK3438 

1500L

300

What are is the name of the bottle used in fill? 

What is the fill volume control limit

HDPE

≤0.8L

400

How many membranes are installed on UF1140, UF1150, UF1063, and UF1160

12, 12, 10, 10

500

Name all the phases for Chrom day 

Equilibration 

transfer in 

Load

wash

Elution 

Regeneration 

sanitization 

storage

Suite J

100

What is the maximum room occupancy during critical operations 

10 people 

200

How many membranes are installed on UF1062 at the 35L, 37.6 and 112.8L scale 

10, 10 and 6

300

What IVT reagent cleaves DNA into smaller pieces and what subsequent step would remove these smaller pieces. 

Dnase 1 and the diafiltration phase 

400

What is the name of the transfer panel in Suite J

TPNL 1110 

500

Name all potential phases that can be run during UF day (including post ops) 

Equilibration 

Diafiltration 

Concentration

Wash (Product recovery)

Flush to Drain (Flush A/B)

Sanitization

Integrity Test

Storage 

Misc

100

what is the exposure time for settling plates (min and max) 

30 minutes to 4 hours

200

What is the expiry for the following items: 

Trigger sterahol

pH 7

CIP

Vesta-syde RTU 

1 month

30 days

4 months

21 days

300

Name 6 covid variants manufactured in GH or J 

WT, BA.1, BA.2, BA 4/5, WT b7, BA 4/5 B7, XBB.1.5, XBB.1.6 spike +, JN.1, KP.2, LF.7, LP.8.1, LP.8.1 b8, BA.3.2.2

400

Name all of the Storage Types (SAP) that belong to Suite GH

GHF, GHS, GHP, GB1 (bonus 100 points for guessing GB2)

500

What are the passing criteria for aseptic qualification 

environmental plates = <1 CFU 

Personal plates = ≤3 CFU 

TSB Broth: No turbidity (growth) 

Impossible

100

How is the date of manufacture generated? 

Start date of final filtration 

200

Building B uses CHO cells. What does CHO stand for?

Chinese Hamster Ovaries 

300

What year of Windows does Suite J currently use for its MCS 

2019

400

What is the size of the mRNA in Suite J? 

350 kda

500

What is the first major process step of GH, J and the fill/finish site? 

GH: Thaw/Seed lab

J: IVT 

Fill/Finish: Dilution (will accept thaw if 3+ failed answers too)