Show:
Take Your Mark
Where in the World
What's the Evidence
T cell Detective
100

I express Cd3 

What is a T cell 

100

CD3⁺ CD8⁺, CD69 low, CD25 low, Ki-67 low, Granzyme B low

What is resting/naïve T cel 

100

You claim that an immune cell is a T cell. Which marker from our lesson provides the strongest evidence?

What is CD3?

100

You know a cell is CD3⁺, but you want to identify its major T-cell lineage. What should you examine next?

What is CD4 and CD8 

200

CD4 and CD8 are most useful for determining this feature of a T cell.

What is a T cell lineage/subset 

200

CD3⁺ CD8⁺, CD69⁺, CD25⁺, Ki-67 low, Granzyme B low

What is activated memory T cell. 

200

You want evidence that activation has begun. Name one marker you could examine.

What is CD69 or CD25

200

A CD8 T cell is CD69⁺. What additional marker from the lesson could provide more evidence of activation?

What is CD25/CD69/CD44

300

CD69, Cd44 and CD25 would fall into this marker category.

What are activation markers?

300

CD3⁺ CD8⁺, CD69⁺, CD25⁺, Ki-67⁺, Granzyme B low

What is an activated/proliferating T cell


300

You believe you T cell is exhausted, what is a marker you can look at to determine this? 

What is PD-1?

300

A T cell is CD69⁺ and CD25⁺. A student says, “That means it is cytotoxic.” What proteins should you examine before accepting that conclusion?

What are Granzyme B and/or perforin?

400

A cell expressing Ki-67 provides evidence that this biological process is occurring.

What is proliferation? 

400

CD3⁺ CD8⁺, Granzyme B⁺ and perforin⁺

What is a cytotoxic effector like T cell


400

You think a T cell is actively proliferating. Which protein would support your claim?

What is Ki67?

400
You notice that your T cells are producing less GranzymeB and Perforin, what is something you could look at to help determine what state they are in? 

What is PD-1?

500

Granzyme B and perforin provide evidence that a CD8 T cell has acquired this type of machinery.

What is a cytotoxic/ effector machienery 

500

CD3⁺ CD8⁺, CD69low, CD25⁺, Ki-67low, Granzyme Blow, PD1+

What is an exhausted T cell?

500

You think a CD8 T cell has acquired cytotoxic machinery. Name one protein that supports the claim.

What is Granzyme B or Perforin? 

500

You are given only “CD3⁺ CD8⁺.” Explain why that isn't enough information to decide whether the cell is resting, activated, proliferating, or cytotoxic.

Because CD3/CD8 establish identity and lineage, but markers of activation, proliferation, and effector function are still needed.