The Binding of Isaac

Characters

100

The main character of the Binding of Isaac.

Isaac

(Wow... Who would have guessed...?!)

200

This character is unlocked after having 55 coins held in one run. They start with two red hearts, one key, and Lucky Foot.

Cain

300

This character is unlocked after making three Devil Deals in one run. Playing as them, the player starts with three black hearts, flight, and a worse Brimstone.

Azazel

400

After beating Isaac (Boss) as ??? (Blue Baby), Isaac (Character) will start future runs with this item.

D6

500

The number of playable characters in base Rebirth.

11

Items and Collectables (See the description, name the item)

100

Reusable pill generator.

Mom's Bottle of Pills

200

Friends 'til the end.

Best Friend

300

It hurts so good +5 bombs.

Pyromaniac

400

He's greedy.

Bum Friend

500

Holy white death.

Crack the Sky

Floors and Rooms

100

When starting a new file, this is the floor you will guarantee start on for a few runs. 

The Basement

200

The only room only has book items in it's item pool.

Library

300

The variant of the Caves floor in Rebirth without DLC.

Catacombs

400

After obtaining the two key parts from killing the Angels, the door to the Mega Satan fight will be open on this floor.

Chest

500

What does the one pip dice room do?

Rerolls the player's current held collectables.

Bosses and Mini Bosses

100

This boss can drop the lump of coal passive item.

Krampus

200

This boss has a varient called the Bloat.

Peep

300

This is the final boss when playing on Ultra Greed mode.

Ultra Greed

400

All of the Horseman Bosses.

Pestilence, War, Famine, Conquest, and Headless Horseman

500

The boss in Corpse II

Mother