Members Past/Present
Navieta-> Jay
Jay->___
Who is:
Tim/Kate/Jackie?
full abbreviation of the "normal" black mouse
What is C57BL/6J?
EconoTaq, check
Agarose Gel, check
100 Digested Tails, check
ZERO expectation of any bands showing up
What is Genotyping/ PCR?
I broke the EPM (again....)
Who is Clay?
Hen Lab Office Space
What is 773? (grad student office)
Currently at UCSF
Giving talk titled "Representing rewarding and aversive experiences in hippocampal circuits"
Tomorrow @3PM
Who is Mazen?
Name of mouse line with a nuclear-localized Cre recombinase inserted just upstream of the first coding ATG of the serotonin transporter gene
What is the Sert-Cre mouse line?
Starved the mice
Grabbed the boxes and lamps
Booked Corner Room
Picked out the most enticing looking food pellets
What is Novelty Suppressed Feeding (NSF)?
The Sharps container is full
The PFA blood container is overflowing
I just spilled something.... I hope this isn't toxic
What is EH&S?
Tris Base for TAE
What is 747? (chemical room, back shelf)
Parents of Lili, Keima, Phoebe (3 answers)
Who are:
Julia, Phi, Grace
NRSA F30, F31, F32, K99, R00, T32
Bonus if you can name them
What are NIH Grants?
Complete TC4750
Frozen Brains
Brand new blade
Labeled well plates
Sebs # on speed dial
What is cryostat cutting?
Protocol expiring in a month
Want to try a new behavior not in protocol
Getting new lab members
Submitting appendices over, and over, and over....
What is IACUC?
PFA for perfusions
Where is 741? (drawer by the PCR boxes)
Former autoclave expert that will also make sure you took a break to eat today
Who is Josephine?
Syn-GCaMP6f.WPRE.SV40
Syn-Flex-ChrimsonR-tdTomato
Syn-DIO-mCherry
What are Adeno-associated Viruses
Book your station
Order your virus 6 months in advance
Grab your STERILE tools and gloves
Pray the mice live long enough for me to image
What is Sterotaxic Surgery?
perfusions are done
staining done
Now I need access to the confocal calendar
Who is Michelle Stackmann?
OFT Boxes
Where is 1063? (oft room)
Members of the Hen Lab Social Committee
Who are Hannah, Carla, Hilledna and Karly?
What are you doing next week
OFT, EPM, ARS, CFC, FST, TST, and maybe SPT if there is time
What are behavior tests?
Come up with novel research question
Design your experiments
Gather your data
Write a compelling summary of years worth of work
Brace yourself for comments from reviewer #2
What is submitting a paper? (or writing a grant I suppose)
Its summertime and I get free lab---- I mean UNDERGRADS.
Gotta email this guy for their onboarding
Who is Wardell Duncan?
(JSYK we appreciate you undergrads!)
The woman who takes ID card Pictures
Where is NOWHERE
"Security desk, 1st floor Pardes"