Initiation
Transcription occurs in what direction?
5' -> 3'
Where does transcription occur in Prokaryotes?
Cytoplasm
What is the gap formed by unwound DNA called?
Transcription bubble
After a eukaryotic cell is done transcribing mRNA, where does the new strand go?
Outside of the nucleus to a ribosome.
True or False: All genes are being transcribed nonstop.
False
What is the strand of DNA called that is being replicated?
Template Strand
Where does transcription occur in Eukaryotes?
Nucleus
Do eukaryotes or prokaryotes transcribe faster?
Prokaryotes
What is required on the mRNA strand to be able to leave the nucleus?
5' cap, removal of introns, addition of a poly A tail
True or false: In prokaryotes, transcription and translation occur at the same time.
True
True or False: Both strands of DNA are transcribed at the same time.
False
Eukaryotes: What regulated the RNA Polymerase II to facilitate transcription?
Enhancer Sequence
Which enzyme surrounds the DNA as it is being replicated?
RNA Polymerase II Complex
What is the matching code of mRNA for this DNA template?
AGCGTTATC
UCGCAAUAG
What are the genes called that ARE being transcribed all the time?
Housekeeper genes
What is the name of the box at the end of the promoter sequence called?
TATA box
Prokaryotes: What is the name of the factor that is associated with RNA polymerase and recognizes the promoter sequence?
Sigma Factor
How many phosphates enter on a ribonucleotide and how many stay attached and build the RNA?
3 enter (ribonucleoside triphosphate)
1 remains (2 get kicked off and release energy)
Is uracil a pyrimidine or a purine?
Pyrimidine
Approximately how many base pairs are in the RNA-DNA duplex?
8
Which enzyme unwinds DNA for replication?
DNA Helicase
Eukaryotes: Which enzyme facilitates local unwinding of DNA and adds nucleotides?
RNA Polymerase II
Channels in the transcription enzyme allow for what material to enter the bubble?
RNA nucleotides
Why is the 5' cap needed before transcription can occur?
The new strand needs the cap in order to get through the nuclear membrane and leave the nucleus.
Introns and Exons: Which groups are removed and which are spliced together?
Introns are removed and Exons get spliced together.