Figure 1
What kind of plot was used in Figure 1A?
How many bacterial families were found to be predominant in the LTS alpha diversity study?
Three
What kind of staining was used in Figure 3A/D?
Immunohistochemical (IHC)
What does LTS-NED stand for?
Long-Term Survival No Evident Disease.
PDAC stands for:
Pancreatic Adenocarcinoma
What were the 2 studied survival groups?
Short-Term Survival (STS) and Long-Term Survival (LTS)
What method was used to create Figure 2B?
LEfSe
What are two T-cell types identified to be in higher concentration in LTS group?
Cd3+ and CD8+
Describe the overall methods of Figure 4B.
Treated mouse with antibiotics, fecal implant from STS donor, injected with PDAC tumor.
What addictive behavior can lead to Pancreatic cancer?
Smoking
What are the abbreviations of the 2 cohorts used for analysis?
MDACC and JHH
True or False: High concentration of the 3 genus families found to lead to Short-Term Survival per Figure 2E.
False, high concentration of the 3 genus families demonstrated Long-Term Survival.
Per Figure 3F, what kind of correlation was found between concentration of each T-cell and Overall Survival?
Positive
Which patient donor appeared to have the highest tumor-suppressing effect on the mouse tumor following FMT?
LTS-NED.
Pancreatic cancer is the ___ leading cause of cancer-related death in the US.
3rd
Which survival group had the highest alpha diversity per Figure 1B?
Long-Term Survival (LTS)
What added bacteria found in LTS potentially influences and predict long-term survivorship in PDAC patients per Figure 2H?
Bacillus Clausii
Based on the analysis of Figure G, which genus had the highest correlation of survival?
Saccharopolyspora
What was the purpose of Figure 4J?
Demonstrate the effects of CD8+ T-cell neutralization on tumor suppression.
What gene mutation is responsible for most pancreatic cancers?
Kras
What were the conclusions of Figures 1D and 1E?
Tumor microbial communities exhibit phylogenetic closeness within each group.
Name 1 of the 2 bacterial families found predominantly in STS cases.
Clostridia or Bacteroidea
What was the overall conclusion of Figure 3?
Tumor microbiome diversity and the present of these 3 genera in the tumor may contribute to he anti-tumor immune response by favoring recuirtment and activation of CD8+ T-cells.
What was the overall conclusion of Figure 4?
Results suggest that gut/tumor bcateria from patients who had PDAC and survived Long-term may have a protective effect against tumors.
What famous gameshow host passed away from Pancreatic Cancer?
Alex Trebek, Jeopardy