Microscopy day 2
Name the parts of the brightfield microscope
Head, arm, and base
Name 3 things in a cell structure
Flagella, Inclusions, plasma membrane
What are the 3 main phases of binary fisson
Growth, DNA Replication, and Cytokinesis
Whats the differnce between endospores and spores
Endospores are a method of survival, and spores are a method of reproduction
Describe what the refractive index does
How much a substance will slow the velocity of light
Which flagella has no tails
Atrichous
What does the lag phase do in bacterial growth
The bacteria is adapting to the new enviroment
What is sepsis
What type of microscope had dark cells and a light background?
Brightfield
What are the steps of gram staining
1. Crystal Violet
2. Iodine
3. Decolorize
4. Counterstain
What is the purpose of antibiotics
They work by blocking something the bacteria are actively doing
Does autoclaving kill endospores?
Yes it kills endospores
What does the transmission microscope view?
internal virus/orgnaells
What shape is a staphylococci
Cluster
Name all the types of temperature bacteria
psychrophile, mesophile, thermophile and extreme thermophile
What does ionizing sterilization do
gamma rays/x-ray, creates a double-stranded break in the DNA. No DNA replication
Describe what a fluorophore is
A fluorophore is a molecule that absorbs light at one wavelength and then emits light at a longer wavelength
What do type 4 pili do
Type IV pili help bacteria attach to surfaces, move (twitching motility), take up DNA, and form biofilms.
What is a facultative anaerobe and an aerotolerant anaerobe?
A facultative anaerobe prefers O2, but is not necessary, and an aerotolerant anaerobe doesn't care about oxygen
How does Algae reproduce
Asexual through fragmentation and binary fission, and sexual through meiosis