Show:
Category 1
Microscopes & a
Bacteria Smear Process
Category 2
Staining Slides
Category 3
History
Category 4
History &
Types of Microbes &
Medias for Growth
Category 5
Medias &
Structure of Bacteria
100
a tool that helps you see small microorganisms that are unable to be seen by the naked eye
What is a microscope? 1-1 Understand that microbiology includes a diversity of microorganisms that require the regular use of a microscope.
100
1. crystal violet dye 2. rinse with water 3. grams iodine 4. rinse with water 5. alcohol, 10-20 seconds 6. safranin 7. rinse with water 8. blot
What is gram staining? Identify the steps of gram staining.
100
plague that killed millions of people prior to having knowledge (through the study of microbiology) about bacteria and other organisms
What is the Black plague? or What is the Bubonic plague? Discuss the historical aspects of the field of microbiology
100
states that microorganisms are the causes of diseases
What is the germ theory? Discuss the importance of the germ theory.
100
a type of media that inhibits the growth of some bacteria while selecting for the growth of others
What is selective media? Contrast selective and differential media.
200
a technique used to increase the resolution of a microscope, achieved by transparent oil increasing the amount of light refractions
What is an oil immersion lens? 1-2 Be able to use correct microscope technique, including the use of the oil immersion lens.
200
grams iodine; intensifies the stain or coats a structure to make it thicker and easier to see after it is stained
What is a mordant? Describe the reason for each step in the process.
200
life can arise from non-living matters; the opposite of biogenesis
What is spontaneous generation? Understand the difference between spontaneous generation and biogenesis.
200
bacteria, algae, fungus, viruses, protozoas, helminths, arthropods
What are microorganisms/microbes? Identify types of microorganisms. (objective is on History of Microbiology & Types of Microbes)
200
a cell that has a nucleus, organelles, a cell wall of cellulose or chitin, goes through mitosis, and has linear chromosomes
What is a eukaryotic cell? Discuss the differences between prokaryotic and eukaryotic cells.
300
uses a beam of electrons instead of light to show internal structures
What is a transmission electron microscope (TEM)? 1-3 Compare and contrast various types of light and electron microscopes and name situations i which each is best used
300
Bacteria are slightly negative, so they are attracted to the positive chromophore of the BASIC dye.
Why does the stain stick to the cell wall? Describe why the stain sticks to the cell wall.
300
1) present in every case 2) taken from host and grown in pure culture 3) reproduced when inoculated into healthy animal 4) recoverable from the infected host
What are Koch's postulates? Describe the importance of Koch's postulates.
300
yeasts, molds, mushrooms
What are fungi (examples)? Identify examples of each type of microbe.
300
a cell that contains appendages (flagella, pilli, fimbriae), cell envelope (capsule, cell wall, plasma membrane), and a cytoplasmic region (DNA, ribosomes, inclusions)
What is a prokaryotic bacterial cell? Identify and describe the parts of a prokaryotic bacteria cell.
400
the part of microscope where the slide of the organism is placed
What is a stage? 1-4 Name the parts and functions of a light microscope.
400
another type of differential stains but one that is used with bacteria that have a lipid based cell wal
What is an acid fast stain? Compare gram stains with acid fast stains
400
found everywhere around us - in the air, water, soil
What is a microbe? Discuss the importance of studying microbes.
400
tetanus, botulism, gonorrhea, chlamydia, tuberculosis, listeriosis, salmonellosis, etc.
What is bacterial diseases? Identify diseases caused by each type of microbe.
400
cocci (round), bacilli (rod), spiral
What shapes do bacteria come in? List and describe the three basic shapes of bacteria.
500
the process of mixing bacteria and water together and then proceeding to flame the slide with the bacteria smear
What is heat fixing? Be able understand and demonstrate the process of heat fixing a slide
500
has a thick cell wall and stains purple
What is Gram (+) bacteria? Identify the basic techniques for identifying bacteria.
500
decreasing contamination by sterilizing instruments and disinfecting dressings
What is aseptic technique? Identify aseptic techniques.
500
a differential bacterial growth medium that can distinguish normal from pathogenic based on the interaction of sheep's blood and bacterial hemolytic enzymes
What is Blood Agar (BAP)? Distinguish between different types of media used to grow bacteria.
500
a type of bacteria that contains an extra outer membrane, the cell wall is thin, and also stain pink
What is gram (-) bacteria? Distinguish between gram positive and gram negative bacterial and discuss the difference between them.