Infections
Media
Guess who
Testing
Virulence
Review
100
common throat infection caused by Group A Strep

pharyngitis

100

hemolysis can be seen on this enrichment media

sheep blood agar

100

Cellulitis

Predictably susceptible to penicillin

Beta

PYR positive & Bacitracin susceptible


Streptococcus pyogenes

100

Antibiotic disk test used to identify Streptococcus pyogenes

Bacitracin

100

breaks down your connective tissue and allows infection to spread

hyaluronidase

100

Catalase positive, microdase negative, GPC in clusters that will clot rabbit plasma

Staphylococcus aureus

200

respiratory infection caused by Streptococcus pneumoniae

pneumonia

200

hemoglobin and NAD are available in this media to help cultivate more fastidious organisms

chocolate agar

200

Neonatal infections

Narrow zone of beta hemolysis

CAMP postive

Streptococcus agalactiae

200

Detects pyrrolidonyl arylamidase and is used to differentiate Group A Strep and Enterococcus species from other catalase, negative GPC

PYR

200

induces antibody response and lyses RBCs, WBCs and platelets

Streptolysin O

200

A dull yellow organism that will produce a positive microdase result and may be seen in tetrads

Micrococcus luteus

300

rare but severe bacterial infection that destroys the tissue beneath the skin, including the fascia and muscles

necrotizing fasciitis

300

agar that inhibits GNR with the addition of colistin and nalidixic acid

Columbia Nalidixic acid agar (CNA)

300

Normal flora of upper respiratory tract

opportunistic

alpha 

Optochin resistant

Streptococcus viridans

300

If the organism can hydrolyze esculin to esculetin, it will react with the ferric iron to form a dark brown-black color

Bile esculin hydrolysis

300

polysaccharides that sits just outside the cell wall and prevents phagocytosis

capsule

300

fully automated microbial identification and antibiotic susceptibility testing system that utilizes special test cards to perform biochemical testing

Vitek

400

inflammation of the protective membranes covering the brain and spinal cord in a neonate

neonatal meningitis

400

broth used for the isolation of Streptococcus agalactiae from vaginal and rectal swabs

Todd hewitt broth

400

Alpha

Lancet

Umbilicated colony

Optochin susceptible

Streptococcus pneumoniae

400

detects specific antigen/carbohydrate on the cell wall of beta hemolytic Streptococci

Serotyping (Lancefield grouping)

400

cytotoxic protein that activates compliment and causes alpha hemolysis

pneumolysin

400

hemolysis, size, form, margin, elevation, color, odor

Gross colony characteristics

500

sequalae autoimmune disease with rheumatologic, cardiac and neurologic manifestations

acute rheumatic fever

500

selective and differential broth intended for the detection of Group B Strep from anovaginal swabs of pregnant women

Carrot broth

500

nonhemolytic or alpha

PYR negative

Bile esculin positive

Unable to grow in 6.5% NaCL

Streptococcus bovis 

500

Demonstrates the hemolytic activity of beta-hemolysin produced by S. aureus, and is enhanced by the extracellular protein produced by Group B Strep

CAMP

500

degrades host DNA/RNA

Streptodornase

500

textbook description of Streptococcus Gram stain

GPC in pairs and chains

600

inflammation of the kidneys filtering unit

acute glomerulonephritis

600

Columbia agar with ribonucleic acid and maltose, used for throat cultures

Streptococcus selective agar

600

nonhemolytic

PYR positive

Bile esculin and 6.5% NaCl positive

VanA

Vancomycin resistant Enterococcus

600

Sodium deoxycholate is used to determine if organism can undergo cell wall lysis

Bile Solubility

600

allows binding to host epithelial cells

fibronectin binding protein

600

cut off small pieces of tissue and touch to slide several times

tissue touch prep