Organelles
Nucleic Acids
Trp
Technologies and Enzymes
CRISPR-Cas9
100

The organelle with the functions of digestion, storage, waste disposal, water balance, cell growth and protection.

What a vacuole?

100

This nucleotide base pairs with thymine in DNA.

What is adenine?

100

The name for a cluster of genes under the control of a single promoter.

What is an operon?

100

The technique involves the processes of denaturation, annealing and elongation.

What is the polymerase chain reaction?

100

The process involving the insertion, removal or replacement of DNA within the genome of a living organism.

What is CRISPR-Cas9?
200

Bound by a double membrane; the inner membrane has a series of folds containing enzymes for ATP synthesis.

What is a mitochondria?

200

This molecule contains uracil and is single-stranded.

What is RNA?

200
One of the eight essential amino acids used to make melatonin and serotonin.

What is tryptophan?

200

The enzyme required to copy DNA, forming a complementary template strand.

What is polymerase?

200
The origin of this process (organism), where it provided immunity against viral infections.

What is bacteria?

300

Involves the synthesis of specific proteins from bound ribosomes.

What is the rough endoplasmic reticulum?
300

A transcript of the specific DNA instruction, a temporary photocopy of the gene.

What is messenger RNA?

300

In the presence of tryptophan, the repressor protein binds to the operator.

When is the operon switched off?

300

The enzyme required to convert RNA into DNA.

What is reverse transcriptase?

300

The molecule that binds to the target sequence, prompting its excision by the Cas nuclease.

What is Guide RNA?

400

The distinguishing feature of this type of cell is its lack of a membrane-bound nucleus.

What is a prokaryote?

400

The molecule that carries amino acids to ribosomes.

What is transfer RNA?

400

In the absence of tryptophan, the repressor protein is dissociated from the operator.

When is the operon switched on?

400

This enzyme's optimum temperature is 75°C.

What is taq polymerase?

400

When changing some of the intended genes, cleavage can occur at unintended positions.

What is an off-target effect?

500

Membrane-enclosed sacs of hydrolytic enzymes, found only in animal cells.

What are lysosomes?

500

The process shown in the diagram.

What is translation?

500

The confusing alternate method of reducing the expression of the trp operon in prokaryotic cells.

What is attenuation?

500

The laboratory technique used to separate and isolate DNA based on their mass/size.

What is gel electrophoresis?

500
The term used when a gene is removed from an organism.

What is knock out?