After an accident where he was illegally using funds to save his dying wife in a cryogenically frozen container, he became frozen himself when he was forced to stop, and eventually turned on Gotham city with the same freezing technology...
Mr. Freeze/ Victor Fries
100
This villain can turn into any shape because he is made up of sand
Sandman/Flint Marko
100
This villain can shape shift into anyone... but in her normal form she is blue..
Mystique
100
The god of mischief
Loki
100
Superman's arch-enemy....he despises Superman because he believes him to be nothing more than an alien that will end up destroying humanity.
Lex Luthor
200
This villain can control plants and uses them to ensnare Batman.
Posion Ivy/ Pamela Isley
200
While trying to grow a new arm, this doctor accidentally transformed himself into a giant reptile
Lizard/ Dr Curt Connors
200
The 'first mutant' who wants to take over the earth
Apocalypse
200
He was the first person to have the serum used on, but it had a negative effect on him due to his evil nature.
The Red Skull
200
This villain wears black and white, is short and has a long nose, almost like a beak
Penguin/ Oswald Chesterfield Cobblepot
300
This villain poisons everyone with a special fear toxin by exposing them to their greatest fears, including Batman.
Scarecrow
300
One of Spiderman's greatest villains, he is a genius who had several mechanical arms attached to his back.
Doctor Octopus/ Otto Ocavius
300
This villain survived the Holocaust, and was once Charles Xavier's close friend.
Magneto/ Erik Lehnsherr
300
This artificial intelligence robot became the greatest threat to the Avengers (apart from Thanos later on)
Ultron
300
One of the most powerful DC villains and the first supervillain to kill Superman.
Doomsday
400
This villain uses elaborate puzzles and riddles with his "Superior Intellect" to try and defeat Batman
The Riddler
400
This villain was once part of Spiderman and is one of his most powerful enemies.
Venom/ Eddie Brock
400
These creations have zero remorse for mutants and humans alike, because they are neither human or mutant.
Sentinel
400
This villain can alter reality and move things with her mind
Scarlet Witch
400
This villain is the sidekick/lover of the Joker, and is completely insane.
Harley Quinn
500
With a love for felines and for stealing priceless diamonds and artifacts, she has also been one of Batman's love interests
Catwoman
500
This villain shoots bolts of electricity as his weapon.
Electro/ Max Dillon
500
He is responsible for giving Wolverine his adamantium skeleton.
William Stryker
500
He is the avengers worst enemy and is trying to destroy the universe using the Infinity Stones
Thanos
500
He is the ruler of the planet, Apokalypse, and is the most powerful enemy of the Justice League.
Hint: His name is also the side of the Force that the bad guys in Star Wars use.