Specimen Handling Basics
Why is proper specimen handling critical for testing accuracy?
It ensures test accuracy by preventing analyte changes and contamination.
What temperature is required for cold agglutinins and cryoglobulins?
37°C (body temperature).
What position should specimens be transported in?
Upright position to promote clot formation
What is one major advantage of POCT?
Faster results for quicker treatment decisions.
What does hemoglobin testing help assess?
Evaluates blood’s oxygen-carrying capacity (for anemia).
Why should blood specimens be gently inverted after collection?
To mix additives and reduce hemolysis.
Why must some specimens be kept at room temperature?
To prevent analyte degradation.
What type of container is used for external transport?
Crush-resistant container with absorbent material.
Why are most POCTs considered CLIA-waived?
They are simple, accurate, and low-risk
Why is glucose testing important for diabetic patients?
Provides immediate feedback for diabetes management.
What happens if blood from different containers is mixed?
It can cause contamination and inaccurate results.
How are specimens kept cool during transport?
Using an ice-water slurry to slow metabolic processes.
Why must couriers transporting specimens be trained?
To ensure safety and prevent contamination during transport.
Give two common POCT tests.
Glucose, cholesterol, cardiac troponin, or hemoglobin tests.
What test measures long-term glucose control?
Hemoglobin A1c test
What type of bag should specimens be transported in?
Leak-resistant biohazard bags with zip closure.
Why are some analytes stored frozen?
Because they remain stable only at −20°C to −70°C.
What PPE does OSHA require during sample processing?
Gloves, lab coat, and protective face gear.
Why is quality assurance important in POCT?
Ensures reliable and valid test results.
What test detects early signs of heart damage?
Cardiac Troponin T or I test
Why is correct labeling essential for specimen integrity?
Prevents mix-ups and ensures traceability.
Give one example of a light-sensitive analyte and how it’s protected.
Bilirubin; Protect with amber tubes or foil.
Why is balancing the centrifuge essential?
Prevents damage and ensures accurate separation of components.
What can happen if calibration and controls are not performed correctly?
Leads to inaccurate results and potential patient harm.
What test detects inflammation or infection using CRP levels?
C-Reactive Protein test