Amino acids
this amino acid is capable of forming disulfide bonds
what is cysteine?
This is the cheapest and most accessible type of microscope
What is a light microscope
Approximately 90% of what we know about cell biology came from studying this number of model organisms.
What is 6?
This group of proteins helps assist with the proper folding of other proteins and helps them to avoid misfolding
What are chaperones?
When a chain of this 76-amino acid-long protein is ligated to another protein, it is tagged for degradation
What is ubiquitin?
The one-letter abbreviation for this amino acid is "K"
What is Lysine?
This type of microscopy requires incredibly thin slices of your sample
What is Transmission Electron Microscopy (TEM)?
This is the common name for the model organism "Mus musculus"
What is mouse?
Infectious prions typically convert alpha helices into this type of secondary protein structure
What are beta sheets?
This type of experiment is used to measure protein half-life and turnover
What is a pulse chase experiment?
These two amino acids are negatively charged
What are aspartic acid and glutamic acid?
This type of microscopy gives high resolution images of surfaces and 3D structures
What is Scanning Electron Microscopy (SEM)?
Both mice and humans have approximately this many genes.
What is 30,000?
During folding, these types of amino acid residues prefer to aggregate together and become "buried" within a protein
What are hydrophobic amino acids?
This is the amino acid residue that ubiquitin can be ligated to.
What is lysine?
This hydrophobic amino acid is found on the N terminus of a protein.
What is methionine?
This type of microscope is able to excite fluorophores with different wavelengths of light
What is a fluorescent microscope?
This is the scientific name for the fruit fly
What is Drosophila melanogaster?
This specific chaperone helps regulate prion formation in yeast
What is Hsp104?
This is the name of the consensus sequence of DNA that Hsf1 binds to
What is the Heat Shock Element?
Phosphorylation can occur on these three amino acids
What are Serine, Tyrosine, and Threonine?
This specific kind of microscope is able to take "layers" of images and stack them to provide a more three-dimensional picture.
What is a confocal microscope?
This term refers to the similarities between the genomes of two species and is an important characteristic of many model organisms.
What is homology?
This chamber-like chaperone protein holds onto polypeptide chains and then "releases" them once they are folded correctly
What is Hsp60?
External stress such as heat exposure results in the phosphorylation and trimerization of this protein, allowing it to translocate to the nucleus and act as a transcription factor.
What is Hsf1?