Proteins
Western Blotting
Life Examples
History
100
This picture is known as the building blocks of proteins.
Szabóová, Andrea, et al. "Searching for Important Amino Acids in DNA-binding Proteins for Histogram Methods." ISBRA 2011 (2011): 48.
What is amino acids?
100
Western blotting test for this macromolecule.
What is proteins?
200
The order of amino acids is determined by this macromolecule
What is DNA?
200
The purpose of adding a secondary antibody is to...
What is to allow detection for the protein of interest?
300
In the picture below, this type of intermolecular force is the reason why these two are shaped like this. 
What are Hydrogen Bonds?
300
what does SDS-Page to proteins?
Hint: 2-part answer from video.
What is denaturing them and covering them in negative charges?
300
The marker bands on the left of the picture are used in reference to helping determine this when the proteins are separated.
Figure 7: HeLa Cell Lysates (LYS001) Separated on a 4-12% Gradient Gel from https://www.bio-rad-antibodies.com/western-blotting-gel-electrophoresis.html?JSESSIONID_STERLING=8D12B1CC8398F856294600B1A97CC7A1.ecommerce1&evCntryLang=US-en&cntry=US&thirdPartyCookieEnabled=true
What is molecular weight?
400
This group is unique to amino acids. They determined ionic bonds, hydrophobic interactions (typically inside of structure), hydrophilic interactions (typically outside of structure), etc. all while driving folding.
What are the R-group (side-chain)?
500
Ionic bonds, hydrophobic/phillic interactions, Van Der Waals interactions, disulfide bonds, etc. all influence what structure?
hint: the last question talked about the importance.
What is tertiary structure?
500
This membrane is capable to bind large amounts of proteins. Its main function is to transfer proteins in the gel to the membrane.
What is a nitrocellulose membrane?
500
Image source: J. Shen, et al. Cancer Res. S64:9018-26 (2004). from website https://www.labxchange.org/library/items/lb:LabXchange:996cc2cf:html:1
In this Western Blot, which antibodies look much more prominent than the other antibodies?
What is Cathepsin D?
*note Cathepsin D has some shifting in its band