Figure 1
the assay that produced figure 1B
what is RNAase protection?
the explanation for the blue stain visible in each L1 C. elegans
what is all nematodes expressed Beta-galactidosidase in early development?
the name of the inserted plasmid
what is pC10L14?
the animal used in the experiment
What is C. briggsae
the number of Lin-14 phenotypes tested
what is three (wt, n355, n536)?
the purpose of the inserted lacZ gene
What is it encodes the Beta-galactidosidase enzyme that reacts with X-Gal to make the blue color?
the purpose of the experiment shown in figure 3
What is to confirm that the same mechanism mediated the temporal down-regulation of the lacZ as the lin-14 mRNA?
the purpose of the top images
What is to confirm the lin-14p temporal down-regulation from L1 to L4 in C. briggsae?
the change in Lin14p gradient from early to late stages in the wild-type lin-14 variable in figure 1A
What is a 10-fold reduction
the significant results of figure 2
what is the wt lin-14 C. elegans with a functioning lin-4 gene was the only condition that had greatly reduced B-galactosidase in L4?
the purpose of myosin
What is the transgenic control?
the three testing conditions
top: wild type C. briggsae
middle: lin-14 3'UTR reporter plasmid
bottom: unc-54 reporter plasmid
the significance of the figure 1B results to the paper hypothesis
What is the similar lin-14 RNA production between early and late stages shows that lin-14 regulation is post-transcriptional?
the reason why the transgene expressed at both L1 and L4 stages with the lin-4(e912) mutant
the lin-4 gene had a null mutation, so there was no down-regulation of the lin-14 gene
the difference in size of the endogenous lin-14 and the transgenic lacZ/lin-14 gene
What is 12 nucleotides? (89 - 77)
the two stains methods used
top photos: goat anti-rabbit flueorescin antibody
middle and bottom photos: B-galactosidase
the number of lin-4 binding sites on the lin-14 phenotype in each column
column 1: seven
column 2: seven
column 3: zero
column 4: two
the difference between lin-14/802nt 3'UTR and lin-14/124nt 3'UTR and their purpose
the 802nt 3'UTR has a 802nt deletion leaving no lin-4 binding sites. the 124nt 3'UTR has four remaining lin-4 binding sites. These two deletions demonstrate the importance of having all seven lin-4 binding sites
the significant results in figure 3, including the transgenic mRNA levels relative to myosin
the ratio of L1 to L4 transcenic col-10-lacZ-lin-14 mRNA was 0.9 relative to myosin. This shows that there was no temporal decrease of the Beta-galactosidase from early to late stages
an explanation for the clear worm next to the stained worm in the bottom L4 photo
What is the worm is dead?