Fixed on fixatives
Solutions used for transporting specimens but do not fix them
What are transport solutions?
This plasma stain will stain erythrocytes bright pink, cytoplasm a medium pink, and connective tissue light pink
What is eosin?
A section for this special stain must be cut at 8-10 microns and the results are amyloid is red, elastic tissue is pale pink, and nuclei is purple/blue
What is congo red?
This is the largest organ of the body and is often identified by the keratinization
What is skin?
This embedding error can be fixed by filling your cassette top with more paraffin
what is exposed ridges/incomplete adhesion?
As cells die due to a lack of oxygen, bacteria within the body begins to breakdown tissue
What is putrification/bacterial attack?
A group that confers the property of color
What is a chromophore?
There are three special stains that can demonstrate acid mucopolysaccharides and one of those stains is alcian blue
what are mucicarmine and colloidal iron?
This tissue is the longest part of the large intestine and therefore plays a part in digestion to reabsorb water, electrolytes, as well as form and store feces
What is the colon?
This section of small intestine has been overstained by a background dye which can be corrected even after coverslipping
What is metanil yellow?
A fixative that penetrates rapidly but leaves tissues soft, lyses red blood cells, and has a PEL of 10 parts per million
What is acetic acid?
The results of this special stain are: DNA is green to blue-green and RNA is rose
What is methyl green-pyronin?
This special stain can use colon, apply or small bowel as a control and its results are acid and sulfated mucopolysaccharides are blue, mucins are blue, nuclei is red, and cytoplasm is red/pink
What is colloidal iron?
This organ serves both endocrine and exocrine functions, exocrine by secreting digestive enzymes into the duodenum and endocrine by helping with the regulation of blood sugar
What is the pancreas?
This section of kidney is displaying a weak reaction due to using old reagents which can easily be fixed by using fresh reagents to get a stronger reaction
What is Schiffs/periodic acid?
A fixative with a ppm of 250 that dehydrates as it fixes tissue but causes extreme shrinkage and over hardening of tissues
What is acetone?
This special stain is used to demonstrate some microorganisms or to differentiate cells in hematopoietic tissue
What is giemsa?
The carbohydrates in the control of this special stain are oxidized to aldehydes which allows them to react with the impregnating solution methenamine-silver solution
What is GMS?
The anterior chamber of the sclera of this is sensory organ contains four prominent layers, episcleral, stroma, lamina fusca, and endothelium
What is the eye?
The nuclei of this section are a reddish-brown instead of being blue like it is supposed to indicating that one step was either not done correctly or something has oxidized
What is bluing/what is hematoxylin break down?
A fixative containing:
2.5g dichromate, 1g sodium sulfate. 100mL distilled water, and 10mL of 37%-40% formaldehyde which is added right before use
What is orth solution?
This reaction is based on the mild hydrolysis of DNA by hydrochloric acid and this hydrolysis generates aldehyde groups to react with Schiffs
What is feulgen?
The results to this special stain are DNA is reddish-purple and cytoplasm is light green, DNA hydrolysis allows the aldehyde groups to react with Schiffs but hydrolysis with hydrochloric acid does not occur for RNA
What is feulgen?
The identifying factor of this tissue is the transitional epithelium, which starts as columnar at the base and at the surface transitions to cuboidal
What is the bladder?
This microtomy error is caused by tissues being too hard, or your knife not being clamped tight enough or your ribbon being too long on your water bath or from your water bath being too hot
What is washboarding?