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PCR and Sequencing
Methods
Cloning
Gene Regulation
Challenge Questions
100
An early method of sequencing developed by the scientist who discovered the 3-dimensional structure of proteins.
What is Sanger Sequencing?
100
This method utilizes agarose and an electric current to detect the relative size and quantity of a DNA sample.
What is gel electrophoresis?
100
The process of introducing a foreign DNA sequence into a host cell for replication and study.
What is molecular cloning.
100
This bacterial cofactor allows RNA polymerase to recognize the promoter region
What is a sigma factor?
100
The type of regulation exhibited by the lac operon.
What is positive regulation?
200
A nucleotide that halts further polymerization due to a missing 3' hydroxyl.
What is a dideoxynucleotidetriphosphate.
200
A method that utilizes DNase I for detecting the presence of DNA binding proteins.
What is DNase Footprinting.
200
A bacterial cell that has uptaken a plasmid.
What is a transformed cell.
200
The generic name of a co-inducer or co-repressor molecule.
What is an effector?
200
The numbers assigned to DNA sequences that allow for quick access in the GenBank database.
What is the accession number?
300
The organism who's DNA polymerase is commonly used in PCR reactions.
What is Thermus aquaticus?
300
In western blotting, this is the name of the protein region recognized by the probed antibody.
What is the antigen.
300
The enzyme that contains the ability to cleave lactose and Xgal.
What is Beta-galactosidase
300
The number of enzymes coded in the trp operon
What is 5 enzymes.
300
The two common properties that separate proteins in a 2-D Gel Electrophoresis.
What is isoelectric points and molecular weight?
400
This cofactor of DNA polymerase is usually included in the PCR buffer.
What is magnesium?
400
Covalently linking DNA with proteins for chromatin precipitation.
What is cross linking?
400
This sequencing/cloning method creates small segments of DNA that can be assembled into a DNA contig.
What is Shotgun Cloning (or Shotgun Sequencing)?
400
The common structure of DNA binding domains.
What is helix-turn-helix
400
The type of BLAST search that allows you to find a protein within the NCBI database using a DNA sequence.
What is blastx?
500
This type of sequencing uses an artificial membrane to detect fluctuations in the membranes current.
What is nanopore sequencing.
500
The commonly used restriction enzyme, found in Rhodopseudomonas sphaerodie, that created blunt ends
What is RsaI?
500
These proteins, which are commonly studies using FISH or other reporter assays, help to control segmentation and structure during development.
What are Hox genes?
500
This molecule is commonly used to induce the expression of the lac operon even when no lactose is present.
What is IPTG?
500
The most abundant transposable element in the human genome. It is commonly used as a marker to study human evolution and population genetics.
What is Alu sequences?