Trouble-shooting
Gram positive cocci
Negative rapid strep
Beta colonies on blood agar
What is Streptococcus pyogenes or positive.
Forward D Typing Components
What are Anti-D and patient RBCs?
Organelle present in both eukaryotes and prokaryotes.
What are ribosomes?
Source of blood agar RBCs.
What are sheep?
Cause for positive test pad and negative control pad of lateral flow.
What is expired or old secondary antibody.
EBV agglutination testing components.
What are
Horse RBCs,Guinea Pig Ag,
Patient serum or plasma
Lyme disease causing bacterium
Micro dilution Testing:
Cloudy: 0.1, 0.2, 0.4, 0.8
Clear: 1.6, 3.2
MIC?
What is 1.6
32 mm measurment
Reference:
31
Interpet.
Susceptible
Anti B: Positive
Anti D: Positive
A cell: Negative
B cell: Negative
Rh Control: Positive
Blood type?
What is discrepant or spontaneous agglutination?
Rapid strep test components.
What are
Throat swab;
primary Ab-conjugate (sample pad)
Primary Ab (test pad)
Secondary Ab (control pad)
Tularemia and rabbits
What is Francisella?
Infection caused by being in a hospital setting
What is nosocomial infection?
A tech applied 95% ethanol for 1 minute during gram staining. What is result of E.coli?
What is no effect?
1. Horse RBCs
2. Patient serum
3. Guinea pig antigen
4. Mix (1) + (2)
5. Mix (1+2) + (3) into quarter sized area.
6. Clear blue background with many clumps.
Interpret.
What is false positive?
Reactive lymphocyte components
What are
1. Large lymphocyte
2. Scalloped edges
4. Blue edges
5. Pulled nucleus or homogenous smooth chromatin
Fungi stain detection method.
Rh Control composition
What is antisera without antibody
Sugars....help differentiate ABO antigens.
What are
H Ag = Fucose
A Ag = GalNAC
B Ag = Galactose
Anti-A: Negative
Anti-B: Negative
Anti-D: Positive
A cell: Positive
B cell: Negative
Blood type. If discrepant provide cause.
Discrepant. Type B but forward not working. Due to no anti-sera added.
2. Plasma cell
3.Antibody
4. Antigen removal. How?
What is
1. Neutralize
2. Opsonize
3. Lysis
Microscopic detection of parasites.
What is trichome stain or iodine wet prep?
3 classes of parasites
What are
Arthropod, helminth, protozoa
Anti-B: Negative
Anti-D: Negative
Ac: Positive
Bc: Negative
Blood type? If discrepant, provide cause.
What is discrepant due to weak antibody?