direction that new DNA is made
What is 5' to 3'?
The shape of DNA is described as this
What is a double helix?
This enzyme binds to the promoter region of DNA to begin protein synthesis (transcription)
What is RNA POLYMERASE?
Used to determine the order of nucleotides in a DNA molecule
What is DNA sequencing?
DNA is a polymer made up of monomers called __________.
What are nucleotides.
Enzyme that joins the Okazaki fragments on the lagging strand
What is DNA ligase?
These are the two steps of protein synthesis. BONUS: Where does each step occur in prokaryotes and eukaryotes?
What are TRANSCRIPTION and TRANSLATION? BONUS: Prokaryotes- both in cytoplasm Eukaryotes- Transcription in nucleus; translation in cytoplasm/ribosome
proteins that help turn specific genes "on" or "off" by binding to nearby DNA, required for eukaryotic transcription
What are transcription factors?
Enzyme that uses RNA as a template to make DNA
What is reverse transcriptase?
This sugar is part of the backbone of the DNA molecule.
What is deoxyribose?
A process used to separate DNA fragments based on size and electrical charge
What is gel elctrophoresis?
Adenine and ___________ are both purines, while cytosine and ___________ are both pyrimidines.
What are GUANINE and THYMINE?
The leading strand will be the complement of this parent strand (you may draw a picture to show)
parent strand will be 3-5'
usually off, but can be turned on when a specific small molecule interacts with a regulatory protein (such as lactose)
What is an inducible operon?
A technique for making many copies of a DNA fragment, involves cycles of heating and cooling
What is polymerase chain reaction (PCR)?