Biochem Test 1 Review

Amino Acids

100

These two amino acids are aromatic and are cut by the enzyme chymotrypsin and have a UV absorbance around 280nm. 

What is F Y W  (Phe, Tyr, Trp)

200

The pka 1 and pka 2 of every amino acid is important for degerming the amino acid from this type of graph? 

Titration curve! 

300

This amino acid has a side chain pka of 8.5 and is one of two amino acids with the element sulfer. 

Cystine 

400

What is the net charge of amino acid W at a pH of 10.5? 

NC = -1 (the COO^- and the NH₂⁰) 

500

The PI of this polypeptide: 

HYGIVK

PI = 9.5 

Secondary Structure Facts

100

What is the pitch of an alpha helix?

5.4 Angstrums 

200

What are the phi and psi angles of Beta Sheets? 

approximately +180 and -180 respectively. 

300

What bond is used to determine  heavy metal posing and is found in tertiary but not quaternary structures? 

Disulfide Bonds 

400

What is the repeating structure of silk Fibroin? 

-Gly-Ser-Gly-Ala-Gly-Ala repeating!! 

500

What are the primary bonding interactions for the following structures?

Primary: ? 

Secondary: ? 

Tertiary: ? 

Quaternary: ? 

Primary: Covalent Bonds 

Secondary: H-Bonds 

Tertiary: Hydrophobic effect 

Quaternary: Hydrophobic effect (No disulfide bonds) 

Separation Techniques

100

What charge is a CM column? 

Negatively charged! (this means that negative and 0 will elute first TOGETHER!!) 

200

What will come out first in a size exclusion column?  

The largest elute first 

300

What two separation techniques will have a log relationship? 

Gel Filtration Chromatography and SDS Page 

400

What do the following blotting techniques do? 

Western? 

Southern? 

Western: Moves proteins from a gel to a membrane.

Southern: Moves DNA from a gel to a membrane.  

500

What comes out first in a REVERSE phase HPLC? 

The most hydrophilic (or most polar molecule) Need to know the polarity of the AAs!!! 

Protein Sequencing

100

What can NOT be the next AA for Chymotrypsin, Trypsinogen, or elastase?  

Proline 

200

What is an N-Terminal reagent used in Edman Degradation?  

PITC (What conditions are needed acid/basic and for which steps?) 

300

What does an invariant residue mean? 

The AA does not change at All!! 

400

What are the two ways to cleave Disulfide bonds? 

1. React with excess thol and cap with Iodoacetic acid

2. Oxidize with performic acid 

500

What do the following things cut after? 

Chymotrypsin? 

Elastase? 

Trypsinogen? 

Cyanogen Bromide? 

Chymotrypsin: F Y W 

Elastase: G A S V 

Trypsinogen: K R 

Cyanogen Bromide: M

Nobel laureates

100

This man won the Nobel Prize for sequencing bovine insulin and for his work in DNA sequencing! 

Frederick Sanger: He won TWO Nobel prizes. 

200

What elution technique did we talk about that won a Nobel prize.  

Cryo-electron microscopy

300

These two men are founders of structural biology and discovered complex secondary structures. 

Linus Pauling and Robert Corey

400

Won the Nobel Prize for discovering prions as a new form of biological infection. 

Stanley Prusiner

500

Won the Nobel prize for denaturation and renaturation of ribonuclease A (RNase A). 

Christian Anfinsen