Hemolysis
General Media
Lactose Fermentation
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100

A clear zone of hemolysis indicated a __________hemolytic organisms.

beta

100

The essential nutrients present in media can be abbreviated CHNOPS, what does the C stand for?

carbon

100

This media has a purple tint to it and is selective for Gram negatives.

MacConkey

100

What kind of hemolysis is this?

gamma

100

This media is best for growing fastidious organisms.

Chocolate agar

200

CNA can show hemolytic patterns and is also selective for which organisms?

Gram positive

200

What is the jelly-like component present in culture media?

agar

200

This media turns yellow when a lactose fermenter grows on it.

Hektoen enteric

200

What kind of hemolysis is this?

beta

200

This media has so many antibiotics in it that only organisms like N. gonorrheae can grown on it.

MTM

300

If an organism has a green color surrounding the colonies on a blood agar plate what kind of hemolysis would this be?

Alpha hemolysis

300

Define general media.

Media that has all the essential nutrients that will not inhibit growth.

300

S. epidermidis turns tan on MacConkey agar because it doesn't ferment lactose.  True or False?

False.

300

What are two facts about the metabolism of this organisms?

NLF and H2S+

300

We plated P. mirabilis and P. aeruginosa on HE media, which one makes black colonies?

P. mirabilis

400

In which kind of hemolysis are bred blood cells completely destroyed?

Beta hemolysis

400

Why do we use agar instead of gelatin or potato for media plates?

Agar is not degraded by microorganisms and stays solid at room temperature.

400

E. coli has pink colonies on MacConkey agar and yellow colonies on Hektoen Enteric because it is a strong lactose fermenter.  True or False?

True

400

Give the kind of media and a fact about the metabolism of this organism.

MacConkey NLF

400

What color would P. aeruginosa colonies be on a MacConkey plate?

tan

500

What is the virulence factor that allows an organism to perform hemolysis?

Hemolysins

500

Who came up with the idea to use agar?

Fannie Hesse

500

If you were to classify Hektoen enteric media using the terms general, selective, or differential which would apply?

Selective for Gram negatives and Differential for lactose fermentation and H2S.

500

Give the name of the media shown here and 2 facts about the metabolism of this organism.

HE NLF H2S-

500

Does E. coli grown on CNA plates?

no

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