Restriction Enzymes
Lab Techniques
Genetic Engineering
Random Biotech
Random
100

Enzyme that cuts a specific nucleotide sequence is a

Restriction enzyme

100

What is the charge of the electrode pole where the wells are at and DNA mixture is inserted, and what is the charge of the electrode pole where the DNA moves towards on the opposite end?

Negative, positive

100

Changing an organisms DNA to give that organism a new trait is known as

Genetic engineering

100

Give two examples of how genetic engineering is used

Plants, medicine, vaccines, giving organisms new traits, insulin, etc., answers will vary

100

According to Indeed, what is the highest paying profession?

Cardiologist

(350k)

200

Why would scientists want to use restriction enzymes?

Because a whole chromosome is too long for scientists too study one particular gene easily

200

What is a restriction map?

Shows the length of DNA fragments between restriction sites in strands of DNA

200

Explain why binary fission is an example of cloning

Because binary fission is asexual reproduction where the cell reproduces by cutting itself into two 

200

What kind of restriction enzyme cut is commonly used in biotechnology?

Sticky ends

200

What is the most popular Valentine's candy in Virginia?

Candy hearts

400

What is a restriction site?

The sequences of nucleotides that are identified and cut by restriction enzymes/the fragments that are cut

400

Which lab technique produces millions, even billions, of copies of specific DNA sequences in just a few hours?

Polymerase Chain Reaction (PCR)

400

Why is genetic engineering possible?

Because the genetic code is shared by all organisms

400

What is a DNA fingerprint?

Individuals DNA that can be used to identify a person at the molecular level

400

What year was the first box of chocolates introduced?

1861

800

What is the blunt end vs sticky end?

Blunt end is a straight cut across the two ends of DNA, sticky ends are staggered cuts with 'hooks' on each end

800

What kind of DNA takes genes from more than one organism- typically using a bacterial plasmid?

Recombinant DNA

800

What are plasmids?

Closed loops of DNA that are separate from the bacterial chromosome and replicate on their own within the cell

800

What do the bands of DNA in gel electrophoresis represent?

The length of DNA fragments in base pairs

800

How many states have a city named Valentine?

(Arizona, Nebraska, Texas, Virginia)

1000

What is CRISPR?

Allows scientists to choose which fragments they want to cut 

1000

This is in PCR-is a short segment of DNA that acts as a starting point for each new strand

Primer

1000

Once the host cell is used for the recombinant DNA, how is the new DNA bonded together to the host DNA? *think enzyme*

DNA ligase (or a process called ligation)

1000

Briefly explain the three steps of Polymerase Chain Reaction 

PCR step 1: separate the double stranded DNA into single strand. Step 2, primers bind to their complementary DNA sequences. Step 3, container heats once again and DNA polymerase begins to build new strands of DNA until the entire strand has been copied. 

1000

According to Billboard, what is the most popular song for the winter?

The neighborhood 'Sweater Weather'

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