Where does DNA store information?
in the sequence of the base pairings
What are the base pairings in RNA?
A-U; C-G
What types of cells does mitosis use to divide?
somatic cells
What kind of bond connects base pairings?
hydrogen bonds
What is the central dogma?
DNA->RNA->Protein
What 4 features define the DNA structure?
right-hand chirality, anti-parallel strands, major and minor grooves, double helix
What are the 3 kinds of RNA?
mRNA, rRNA, tRNA
How do bacteria cells replicate?
binary fission
What are the three components that make up a nucleotide? What about a nucleoside?
sugar, base, phosphate; sugar and base
Who discovered the structure of DNA?
Watson, Crick, and Franklin
In what direction is DNA replicated? What direction do you read DNA?
5'-3'; 3'-5'
What is the process of turning RNA into Proteins?
translation
List the 4 stages of the cell cycle:
G1, S, G2, M
What bond connects two nucleotides together?
Phosphodiester bonds
What does Chargaff's rules state?
For every A there is an equal amount of T; for every G there is an equal amount of C
If the DNA strand is 20% A, what percentage does G make?
30%
Transcribe this DNA strand (remember what direction RNA is made!):
3'-TACGAACTCGGCATTCAAG-5'
5'-AUGCUUGAGCCGUAAGUUC-3'
List the 4 phases of mitosis:
Prophase, Metaphase, Anaphase, Telophase
What bases are pyrimidines? What bases are purines?
T, C, U; A, G
List the start and stop codons:
AUG, UAG, UGA, UAA
Replicate the strand (remember what direction DNA is made!):
5'-ATTAGGCACTGCCATT-3'
5'-AATGGCAGTGCCTAAT-3'
Translate this RNA strand:
5'-AUGCUUGAGCCGUAAGUUC-3'
MET LEU GLU PRO Stop
Before DNA replication, a diploid (2n) frog cell has 24 chromosomes (2x12). How many chromosomes does the frog have during S-phase? How many sister chromatids does the frog have after S-phase?
24; 48
What ends does the phosphodiester bond connect the two nucleotides together?
3' carbon on the sugar to the 5' phosphate group
What is the difference between genotype and phenotype?
Genotype is the genetic code and phenotype is the physically expressed trait