This petite, brunette Jedi Master in the Weaver lab holds dominion over training and scheduling for the EV Center's ZetaView NTA.
Who is Cara Bekas?
This is the supernatant containing EVs and other secreted factors that we collect from cells in culture.
What is conditioned media?
Hardin, Heuser and Stahl first described release of exosomes from this endosome-derived organelle.
What is the multivesicular body?
Of this class of membrane-bending transmembrane proteins, CD63 is most highly enriched in exosomes.
What are tetraspanins?
These large EVs are released from cells undergoing programmed cell death.
What are apoptotic bodies?
This high tech wizard of lipidomics and RNA-seq is friend to two and four legged creatures alike!
Who is Kasey Vickers?
You have a need for speed if you want to separate large EVs, small EVs, exomeres and supermeres using this method.
What is differential centrifugation?
Exocyst-containing ectosomes are shown here budding from these microtubule-based antenna-like organelles.
What are cilia?
Hrs is a subunit of this machinery, which recognizes ubiquitinated proteins and targets them for incorporation into ILVs.
What is ESCRT (ESCRT-0)?
Fragments and even wholly intact examples of this energy-producing organelle have been revealed inside various types of large EVs by electron microscopy imaging.
What are mitochondria?
According to this pathology and immunology phenom, we wheeze and sneeze and … release EVs!
Who is Heather Pua?
This technology tracks the Brownian motion of individual particles to determine EV size and concentration.
What is Zetaview/nanoparticle tracking analysis?
You can take pictures like this of cargoes on individual EVs using this piece of EV Center equipment.
What is the ONI Nanoimager?
The term “exosome” was first co-opted by this pioneer of the EV field, who once described her scientific journey as “Alice in Blunderland”.
Who is Rose Johnstone?
These uncommonly large EVs are associated with a highly metastatic “amoeboid” tumor cell phenotype.
What are large oncosomes?
This EV Engineering Wizard and Time-Traveling Mad Scientist share a passion for metabolic engineering, biofuels and flux analysis!
Who are Jamey Young and Doc Brown?
These super-light particles may still be floating in your supernatant after you isolate EVs from cell media.
What are supermeres?
This electron micrograph depicts the cup-like shape of these endosomally-derived small EVs.
What are exosomes?
Publishing within a week of each other in 1983, two independent studies of this transport protein led to the discovery of exosomes.
What is the transferrin receptor?
Ectosome budding requires molecular rearrangements of the plasma membrane and this underlying structural network.
What is the actin cytoskeleton?
This high-tech Phenom is the Olympic gold medalist of Tweezing EVs !
Who is Justus Ndukaife?
EV fingerprinting uses this fluorescent fluidics analysis to classify EV subpopulations.
What is flow cytometry?
These small but mighty nucleic acids can hitch a ride in EVs to alter gene expression in far-off recipient cells
What are miRNA?
Rab27a is required at this step in exosome biogenesis involving the MVB and the plasma membrane.
What is MVB docking?
This ESCRT protein is recruited to the plasma membrane by ARRDC1 to generate small microvesicles called ARMMs.
What is TSG101?