CHEM
BIO- CHEM
LABS
MICROBES
BACTERIA
100
Which of the 4 bonds are broken by boiling temperatures?
Ionic, Hydrogen bonds, & Hydrophobic interactions.
100
What does CHNOPS stand for?
Carbon, Hydrogen, Nitrogen, Oxygen, Phosphorus, & Sulfur.
100
Draw an example of the streak plate, properly labeled!
Use your imagination for the specific bacteria and media type :)
Must include:
Bacteria name
Name/ Initials
Date
Media type
Group#
100
What is a microbe?
Microscopic Organism
100
Which DOMAIN do bacteria belong to?
BACTERIA
200
What are the DNA and RNA base pairs?
DNA:
Adenine- Thymine
Guanine- Cytosine
RNA:
Adenine- Uracil
Guanine- Cytosine
200
1) Identify this reaction and then...
2)Draw a Hydrolysis Reaction with this information...
1) Dehydration Synthesis
2) Maltose + Water ->Glucose + Glucose
200
What is the difference between chemically defined media and complex (rich) media?
In chemically defined the EXACT composition is known.
200
Which identification method was used to create the three domain system?
Nucleic Acid Testing.
200
Give an example of a species of bacteria.
Written using both the Scientific name and correct shorthand.
HINT: Include genus and species name
Streptococcus pyogenes
S. pyogenes
(Should use underline only when HANDWRITTING)
300
Explain the difference between the forces holding water MOLECULES together and the forces holding the hydrogen and oxygen ATOMS together.
BONUS: Which are being broken by boiling?
Polar covalent bonds & Hydrogen bonds.
300
Why is the polarity of water important to microbial life?
Universal solvent...
300
List 4 ways to prevent contamination.
Covering the plate, working close to the flame, wearing PPE, dehydrate loop, flaming the loop between every step, flaming the test tubes, opening/pinky technique...
300
List 3 differences between a Virus and a Bacteria.
Reproduction methods, metabolic abilities, size....
300
How do bacteria reproduce?
BONUS: How does that differ from eukaryotes? virus?
Binary Fission
Mitosis & Hi-jacking of the host mechanical systems.
400
Describe 3 differences between saturated and unsaturated fats.
Saturated:
straight, stack, solid at room temperature.
Unsaturated :
Bent, move around easily, liquid at room temperature.
400
What are the 4 levels of protein structures and which types of bonds hold each type of structure together?
Primary- Covalent (Poly peptide bonds)
Secondary- Hydrogen Bonds
Tertiary- All 4-(Ionic, Hydrogen bond, Covalent, & Hydrophobic Interactions)
Quaternary- All 4 (Ionic, Hydrogen bond, Covalent, & Hydrophobic Interactions)
400
Which pipette should you use to transfer 250 μL?
Bonus points:
Convert to nL & mL
1,000 μL pipette
.25 nL
250,000 mL
400
List three differences between eukaryotes and prokaryotes.
Eukaryotes:
nucleus
membrane bound organelles
5-100 μm
Prokaryotes:
No nucleus
No organelles
only about 1 μm wide..
400
List the shapes of bacteria.
Bacillus
Coccus
spirochete
500
List the 5 specific types of bonds/interactions we have been learning. One way you identify each type on the skeletal structures.
Ionic- +/-
Hydrogen- Dotted line
Polar Covalent- Solid line- Nitrogen or Oxygen
Non-polar Covalent- Solid line- NO NO
Hydrophobic Interactions- Two molecules next to each other W/ both non-polar covalent bonds.
500
List an example of where one would find each CHNOPS element within a microbial structure.
Carbon- Organic molecules, proteins, nucleic acids, carbohydrates, lipids...
Hydrogen- Organic molecules, proteins, nucleic acids, carbohydrates, lipids...
Nitrogen- Proteins, nucleic acids..
Oxygen- proteins, nucleic acids, carbohydrates..
Phosphorus- Nucleic acids, phospholipids
Sulfur- proteins
500
Write your own experiment using Scientific Method.
HINT: There are FIVE PARTS!
Question
Hypothesis
Experimental control
Positive control
Negative control
500
Recreate your concept map without looking including the terms:
Microbe
Virus
Eukaryote
Prokaryote
Bacteria
Protist
Archaea
Fungus
Cellular
Acellular
Gram-Positive
Gram-Negative
Microbe
Acellular-virus
Cellular:
Prokaryote- bacteria & archaea (bacteria- + & -)
Eukaryote- Protist & Fungus
500
Describe the differences between gram positive and gram negative cell walls.
EXTRA BONUS: Draw a picture of both cell walls.
Positive:
stain purple
Plasma membrane, periplasmic space, thick peptidoglycan layer, w/ teichoic acid.
Negative:
stain pink
Plasma membrane, periplasmic space, small peptidoglycan layer, lipopolysaccharide layer.