What am I?
10% aqueous formalin is made up of 100 mL of 37-40% formaldehyde and this
900 mL of distilled water
Bouin solution
Picric acid, 37-40 % formaldehyde, glacial acetic acid
Oil red O requires this type of mounting media
The two major problems with fixation are delayed fixation and this
Incomplete fixation
Unfixed tissue that needs to be held for several days or transported over a long distance should be placed in this
Michel transport medium
37-40% formaldehyde, ethyl alcohol and distilled water
Clarke solution is made of this
Absolute alcohol and glacial acetic acid
Plasma cell cytoplasm is stained this color using the methyl green-pyronin technique
Red-rose
Autolysis is caused when this happens
Formaldehyde, glutaraldehyde, and Zamboni solutions can be used for light microscopy and this
Electron microscopy
Mercuric chloride, sodium acetate and distilled water are my stock solution. When mixed with formaldehyde I become a working solution
B-5
Zenker working solution
Stock- Mercuric chloride, potassium dichromate, sodium sulfate, distilled water
+ Glacial acid acid
This hematoxylin solution must be used within a few days after being prepared
Weigert- ferric chloride will over oxidize due to being a strong oxidizer
Smudgy nuclei and nuclear bubbling can result when this occurs
Incomplete fixation
Specimens remain in fixative solutions are room temp indefinitely, penetrate quickly, and can fix tissues for light and electron microscopy are all advantages for this group of fixatives
Primary buffered PAF fixation (Zamboni solutions)
Mercuric chloride, potassium dichromate, sodium sulfate and distilled water are the stock solution for these
Zenker and Helly
Methacarn substitutes this for ethyl alcohol in Carnoy solution
Methyl alcohol
In regressive staining hematoxylin is overstained and removed by this process
Differentiation
If the tissue is not well fixed when processing has begun, fixation continues in this causing the center to become more eosinophilic than periphery
This is a nonaqueous fixative that is frequently used on frozen tissue sections for IHC
Acetone
Copper acetate, picric acid, formaldehyde 37-40%, acetic acid and distilled water make up this fixative solution
Hollande solution
Gendre solution
95% alcohol saturated with picric acid, 37-40% formaldehyde, glacial acetic acid
Treatment in a weak basic aqueous solution like ammonium hydroxide that changes the dye from red to blue is called this
Bluing
Lugol iodine solution can remove this
Mercury pigments
The fixative solution is preferred for viewing chromaffin granules in the cytoplasm of the adrenal medulla
Orth solution