Biotechnology
The manipulation of organisms or their components to make useful products.
What is biotechnology?
To carry the gene into the host nucleus.
What is a vector used for?
Restriction Enzymes
What enzyme is used to cut DNA?
Fill in the Blank:
A plasmid is a type of ______ commonly used in genetic engineering.
What is a vector?
PCR stands for:
What is Polymerase Chain Reaction?
Manipulation of DNA, cloning, recombinant DNA, gene therapy, production of insulin and growth hormones, stem cell research, gene mapping, DNA sequencing, forensics.
What are some examples of biotechnology used today?
Plasmids and Viruses.
What are vectors commonly used in genetic engineering?
Short extensions of exposed nucleotides from restriction fragments.
What are sticky ends?
Plasmids replicate in one of two ways: independently or dependently...
What is independently?
A method for making many copies of a specific segment of DNA.
What is the process of PCR?
I can prevent diseases before they even happen.
How does biotechnology affect us?
A cloning vector is used to insert foreign DNA into another cell and create multiple copies of the same.
Why are vectors important in cloning?
Segments created by the cutting of DNA with restriction enzymes.
What are restriction fragments?
A way to get genes into bacteria easily.
How can plasmids help us?
Heating, Priming, Copying, Repeat
What are the 4 steps for PCR?
It can make farming more profitable by increasing crop quality.
How does biotechnology affect crops?
Why are vectors important in real life?
They have many real-life applications, including situations involving force or velocity.
An enzyme that can join the sticky ends of DNA fragments.
What is DNA ligase?
To carry antibiotic resistant genes and spread them throughout the body.
What is the primary function of a plasmid?
To amplify the identified disease region.
How is PCR used in the diagnosis of diseases?
Could cause harm to the environment and GMO's in crops.
What is a big concern with biotechnology?
Animal that causes a lot of problems to humans because they may spread many unwanted parasites and disease.
What is the mighty mosquito?
Where are restriction enzymes are generally extracted from:
What is in bacteria?
Bacterial Transformation
How do we get the recombinant DNA plasmid into the bacteria?
1. Only a tiny amount of DNA needs to be present in the starting material.
2. Make copies of very rare DNA.
3. Detect genes of pathogens in infected cells or biological weapons.
What are the advantages of PCR?