DNA Extraction & PCR
Pure DNA has a ratio of approximately
1.8-2.0
Short arm
HLA
Human Leukocyte Antigen
The use of pronase in a flow crossmatch assists in removing non-specific binding due to Fc receptors, as well as
Removing CD20 from the cell surface
PPE stands for
Personal Protective Equipment
_________digests cellular proteins released following lysis.
Proteinase K protease
HLA Class I are ____,____, ____ and HLA Class II are ___,___,____
Class II: DR, DQ, DP
MHC
Major Histocompatibility complex
Ficoll-Hypaque separates lymphocytes based upon what inherent property?
Density
HLA is inspected and accredited by which agencies
ASHI & CAP
also NYS
Which of the following is the preferred sample for DNA extraction?
a. Whole blood without anticoagulants
b. Red blood cells
c. Heparinized serum
d. Platelets
e. Whole blood with ACD anticoagulant
e. Whole blood with ACD anticoagulant
______ are the building blocks that are incorporated into the DNA chain during PCR
dNTPs
WHO
World Health Organization
High resolution Class I and Class II typing is most important in which of the following situations?
a. Living-related kidney transplantation with high PRA
b. ABO-mismatched liver transplant
c. Unrelated hematopoietic stem cell transplantation
d. Autologous hematopoietic stem cell transplantation
e. Two haplotype matched living related hematopoietic stem cell transplantation
c. Unrelated hematopoietic stem cell transplantation
12
The absolute A260 and A280 values and their ratio for a DNA sample provide what information?
RNA/Protein contamination of DNA
Donor ABO- O
CREG
Cross-reactive epitope groups
family of HLA antigens that share some, but not all, of the polymorphisms. For Solid Organ transplant, CREG matches are less likely to be immunogenic because of the shared epitopes (the amino acid motif which is recognized by an antibody)
The structural components of a nucleotide are…
Base, Sugar, Phosphate
NAME 3 of the 6 elements a person is evaluated on to determine competency in a test system
Direct observation of routine test performance
Direct observation of instrument maintenance and function
PT or blind sample
problem solving skills and knowledge
Review of records, QC, preventative maintenance and worksheets
Monitoring entry of information into LIS
In a reverse SSO assay, the genomic DNA is often incubated with sodium hydroxide before application to the membrane. Why?
To denature the DNA
It is acceptable for a red top to be collected from a patient who is at that moment receiving a dialysis treatment
false
PRA
Panel Reactive Antibody;
Indicates the probability of a Positive XM (percentage of donor pool that is predicted to be positive)
Molecular typing usually uses genomic DNA as a starting material. If RNA is converted into cDNA for typing, what feature is missing that may require adaptation of methodology from genomic DNA typing? a. Base m
Introns
Which of the following activities should be performed in the post-PCR area?
a. Amplification, probing, high stringency washes
b. DNA extraction, low stringency wash, probing
c. Gel electrophoresis, probing, primer aliquoting
d. Amplification, probing, primer aliquoting
e. DNA extraction, high stringency wash, primer synthesis
a. Amplification, probing, high stringency washes