Page 1
What is the optimum incubation temperature and atmosphere for most micro cultures?
35oC, with 5%CO2, with ADDED HUMIDITY for 24-48 hours
Crystal violet
What would happen if you left the decolorizer on too long?
Gram positive bacteria would be pink
What is agar?
a seaweed extract used in solid media preparation
Which class of bacteria can grow in aerobic or anaerobic conditions?
Facultative anaerobes
Which type of media contains factors that give colonies distinct, recognizeable characteristics?
Differential media
Name the 3 types of hemolysis patterns and give a brief description.
Alpha (partial)- green
Beta (complete) - clear
Gamma - NO hemolysis
Which genus of organisms are catalase positive?
Staphs
What 2 tests will be positive on the dipstick test of urine that indicate a culture should be done?
nitrite
leukocyte esterase
What type of differential stain is used most often in microbiology?
Gram stain
In the Gram stain, what is the counterstain?
Safranin
What type of culture is made using a specimen directly from the patient?
Primary
What is the generation time for E. coli?
20 minutes
Incubator chambers are ___________ because water is necessary for metabolic reactions.
humidified
What color are lactose fermenters on MacConkey?
purple or hot pink
Which medium is inhibitory?
Selective
Which organism is bile solubile?
Streptococcus pneumoniae
What is used to measure the turbidity of solutions set up for susceptibility testing?
McFarland standard
Which term describes a single species of bacteria that may have 2 different shapes?
pleomorphic
In the Gram stain, what role does alcohol/acetone play?
decolorizer
What type of culture is set up using one isolated colony from the primary culture?
subculture
What must obligate aerobes has for growth?
oxygen
Which bacteria requires the enrichment media Buffered Charcoal yeast extract for growth in culture?
Legionella pneumophila
Name 2 nonlactose fermenters on MacConkey.
Salmonella
Shigella
Which medium is used to preserve a specimen until it gets to the lab?
Transport media
Which test is used to differentiate S. aureus from other staph species?
Coagulase
The disk diffusion method used to determine antimicrobial susceptibility
Kirby-Bauer
Heat
95% Methanol
In the Gram stain, which reagent serves as the mordant?
Gram's iodine
What begins with one single parent bacterial cell and multiplies many times until a visible pile forms?
colony
Which group of bacteria grow best in reduced oxygen?
microaerophiles
Which media contains crystal violet to inhibit gram positive cocci?
MacConkey
Which enrichment media contains heated blood?
Chocolate
Which media could be used to inhibit E. coli?
CNA
Lower UTIs of the bladder or urethra
What is cystitis?
What test can be used to confirm Candida albicans?
Germ-tube
What is the term used to describe the substance that combines with a dye to form an insoluble complex?
mordant
What would happen if you skipped the decolorizer step?
Gram negative bacteria would be purple
What term describes a growth of several colonies orginating from one colony?
Pure culture
Which group of bacteria are oxygen intolerant?
obligate anaerobes
Which media contains antibiotics (collistin nalidixic acid) that inhibit E. coli and other gram negative bacteria?
CNA
What 2 nutrients are found in Chocolate media?
X factor (hemin)
V factor (NAD)
Which enzyme breaks down hydrogen peroxide to oxygen and water?
catalase
Upper UTIs of the ureters and kidneys
pyelonephritis
What is the common name for Enterobius vermicularis?
pinworm