Acid Fast
This stain for bacteria utilizes peanut oil because the use of alcohol for rehydration of slides would cause negative staining due to the target organisms not being alcohol-fast
Fite
Gram + organisms will stain this color in the Brown-Hopps method
Blue-black
This is the recommended counterstain when PAS is used to demonstrate fungi
Light green
This could result in acid fast organisms that appear on slide, but slightly out of the plane of the section
Using contaminated tap water in the water bath
This component of the fungal cell wall makes it PAS positive
Carbohydrates
This fixative should be avoided for acid-fast stains as it could cause normally acid-fast tissues to stain negative
Carnoy
The differential staining achieved with the Gram stain is because of differences in this bacterial quality
Cell wall thickness and structure
This is the preferred fixative for the Warthin Stary technique
10% NBF
This error in the Gram staining process may cause the formation of insoluble compounds that are difficult or impossible to decolorize
Allowing the slides to dry at any step in the procedure
These types of methods would be necessary for the visualization of viruses
IHC (Immunohistochemistry) or ISH (In Situ Hybridization)
Acid alcohol performs this role in carbol-fuchsin techniques
Differentiator
This is the mordant for Gram stains
Iodine
This component differs when comparing PAS to CAS
The oxidizer (periodic acid vs. chromic acid)
This could cause chromic acid stained slides to turn brown
Not completely removing alcohol before placing slides in chromic acid
This step following Schiff reagent helps remove excess leucofuchsin
Rinsing with a sulfite solution
This type of water should be used when staining for acid-fast organisms and in which steps of histology
Millipore-filtered water in the flotation bath for microtomy and in the staining procedure if a water rinse is needed before Carbol-fuchsin is used
This is the name of a specific proprietary formula of the Romanowsky type stain that can be used to demonstrate microorganisms
Diff-Quik
Cryptococcus neoformans is unique compared to other yeastlike fungi due to its mucoid capsule, which means it can be demonstrated by these techniques
Those for acid mucosubstances like Mayer mucicarmine, Alcian blue, and Colloidal iron
This is the process for correcting slides that were overstained with methylene blue in the Kinyoun acid-fast stain
Back the sections up to acid alcohol, then re-counterstain
This specific component in the waxy layer of the bacterial cell wall is disrupted by Carnoy fixative, making acid-fast organisms stain non acid-fast
Mycolic acid
This is the property of microorganisms that acid fast stains depend on
Lipoid capsule and the ability of these organisms to resist decolorization with dilute acid
This category of dyes are used to demonstrate H. pylori
Romanowsky-type dyes
Stains for the demonstration of spirochetes are based on this property
Argyrophilia
Changing the time in this solution would help correct Brown & Hopps Gram stained slides with an intense red background
Picric acid-acetone differentiation
Grocott Methenamine Silver when used on the liver will prominently stain glycogen, causing a diffuse black stain that makes it difficult to see organisms. What could be done to prevent this?
Diastase digestion (Carbohydrates & Amyloids chapter)