DNA
What does DNA stand for
What is deoxyribose nucleic acid?
These three pieces of PPE are always required in the lab
What are gloves, goggles, and lab coat
When do you go down the the first stop?
What is when aspriating
True or false? You should never touch your face or phone with your gloves
This equation is used to dilute liquid stock
What is C1V1
DNA is has what charge?
What is negative?
What is biohaz
True or false? You can set a pipette out of range and it will be totally fine
What is false!
You should always clean with this before starting an expiriment
BONUS QUESTION
Why 70% and not 100%
M=?
What mols/liter
Large pieces of DNA are found where on a gel? Why?
What is Top because large pieces of DNA cannot fit through the microscopic holes in the gel.
True or False: You should always pH with a friend. Why?
What is the range of a p100 pipette
10 uL - 100 uL
How many times should a serological tip be used
What is once
I have a stock of 5M tris buffer. I want 1 liter of 400mM tris buffer. How would I make this?
What is measure 80 mLs of stock in a graduated cylinder and QS to 1 L with water.
What does PCR stand for and what does it do
Polymerase chain reaction, a reaction that copies one strand of DNA into one billion
DAILY DOUBLE
A _____provides detailed information about a hazardous chemical's properties, health hazards, protective measures, and safety precautions for handling, storing, and transporting
Safety Data Sheet (SDS)
How would you measure 657 uL
If you need to put a cap down on you bench which way should you do it?
What is face up?
NaCl FW: 58.44
How would I make this
What is use a scale to measure 233.76 grams of NaCl, stir that with about 500 mLs of water until dissolved, and QS to 1 L.
DNA makes RNA makes ___________
What is protein
Oh no! You spilled a chemical. Whats the first thing you should do?
What is tell a lab manager
How would you measure 7.4 mLs?
What is using a 10 mL serological to measure 7 mLs and a p1000 to measure 400 uL
OR
What is using a 10 mL serological to measure 7.4
What is spray down the cabinet, spray down you arms, and make sure not not block the fan?
I want to make a 1L buffer thats 1M tris and 0.5M NaCl
My tris stock is 5M
NaCl FW: 58.44
How would I make this
What is weigh 29.22 grams of salt on scale. Then dissolve in about 500 mLs of water. Put this in a graduated cylinder. Add 200 mLs of tris stock. QS to 1 L