What Is It?
What does PCR stand for?
Polymerase Chain Reaction
Who discovered it?
Kary Banks Mullis
What are some applications?
Genotyping, cloning, mutation detection, sequencing microarrays, forensics, and paternity testing
What is its function?
It creates multiple copies of a specific segment of DNA
What year was is discovered?
Between 1983-1985
How long did this whole process take?
14 years
How does it work?
Using short fragments of DNA, they rapidly amplify the DNA replication process
How was it discovered?
Kary was trying to improve the synthesis of oligonucleotides. He envisioned the concept of PCR on a camping trip in 1969.
What are the 3 different stages?
1. Denaturing
2. Annealing
3. Extending