500
Describe how to do a gram stain
What is apply sterile water to clean slide, obtain a colony off of agar plate with sterile loop, mix together and allow slide to dry, then heat fix slide.
Apply crystal violet stain, let sit for 1 min, rinse off
Apply iodine, let sit for 1 min, rinse off
With slide at 30-45 degree, apply decolorize for 3-5 seconds , rinse with water
Apply safranin, let sit for 1 min, rinse off, let dry
Examine slide under 100x