Nucleic Acids
DNA Replication
Proteins
Mutations
Separation Techniques
100

Name all of the nitrogenous bases in RNA.

What is adenine, guanine, cytosine, and uracil.

100

Which enzyme unzips the DNA?

What is helicase.

100

What amino acid does the codon AUG code for.

What is methionine.

100

List the three effects a mutation can have on an organism?

What is detrimental effect, neutral effect, and beneficial effect.

100

What is the purpose of PCR?

What is to amplify millions of copies of a specific sequence of DNA.

200

What type of bond makes up the sugar-phosphate backbone?

What are covalent bonds?

200

What is the function of the enzyme topoisomerase?

What is to relieve supercoiling.

200

What are the four levels of protein structure?

What are primary, secondary, tertiary, and quaternary.

200

What type of mutation affects an entire organism and can be passed down to their offspring?

What is a germ-line mutation.

200

What is the name of the polymerase used in PCR?

What is Taq polymerase.

300

Which nitrogenous bases are purines?

What is adenine and guanine?

300

Which direction does DNA Polymerase read the template strand?

What is 3' to 5'.

300

What are the three stop codons?

What are UAA, UAG, and UGA.

300

Provide an example of a transversion mutation.

What is a AT to TA or CG to GC.

300

Calculate the Rf of analyte A.

What is 0.75 (3/4).

400

How many carbon ring(s) do pyrimidines have?

What is one carbon ring.

400

Which direction is the DNA on the lagging strand synthesized?

What is 3' to 5'.

400

During posttranscriptional modification, which segments of RNA are removed?

What are introns.

400

Which type of mutation is most severe?

What is a frameshift mutation.

400

What do all of the letters stand for in the term SDS-PAGE?

What is sodium dodecyl-sulfate polyacrylamide gel electrophoresis.

500

Which end of DNA has a hydroxyl group?

What is 3' end.

500

What is the function of single stranded binding proteins?

What is to stop the separated DNA strands from joining back together.

500

What two things are added to mRNA before it leaves the nucleus?

What are the poly-A tail and the guanosine cap.

500

What type of mutation causes Sickle Cell Disease?

What is a missense mutation?

500

What is the name of the bacteria that Taq polymerase is made from?

Thermus Aquaticus

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