Take Your Mark
I express Cd3
What is a T cell
CD3⁺ CD8⁺, CD69 low, CD25 low, Ki-67 low, Granzyme B low
What is resting/naïve T cel
You claim that an immune cell is a T cell. Which marker from our lesson provides the strongest evidence?
What is CD3?
You know a cell is CD3⁺, but you want to identify its major T-cell lineage. What should you examine next?
What is CD4 and CD8
CD4 and CD8 are most useful for determining this feature of a T cell.
What is a T cell lineage/subset
CD3⁺ CD8⁺, CD69⁺, CD25⁺, Ki-67 low, Granzyme B low
What is activated memory T cell.
You want evidence that activation has begun. Name one marker you could examine.
What is CD69 or CD25
A CD8 T cell is CD69⁺. What additional marker from the lesson could provide more evidence of activation?
What is CD25/CD69/CD44
CD69, Cd44 and CD25 would fall into this marker category.
What are activation markers?
CD3⁺ CD8⁺, CD69⁺, CD25⁺, Ki-67⁺, Granzyme B low
What is an activated/proliferating T cell
You believe you T cell is exhausted, what is a marker you can look at to determine this?
What is PD-1?
A T cell is CD69⁺ and CD25⁺. A student says, “That means it is cytotoxic.” What proteins should you examine before accepting that conclusion?
What are Granzyme B and/or perforin?
A cell expressing Ki-67 provides evidence that this biological process is occurring.
What is proliferation?
CD3⁺ CD8⁺, Granzyme B⁺ and perforin⁺
What is a cytotoxic effector like T cell
You think a T cell is actively proliferating. Which protein would support your claim?
What is Ki67?
What is PD-1?
Granzyme B and perforin provide evidence that a CD8 T cell has acquired this type of machinery.
What is a cytotoxic/ effector machienery
CD3⁺ CD8⁺, CD69low, CD25⁺, Ki-67low, Granzyme Blow, PD1+
What is an exhausted T cell?
You think a CD8 T cell has acquired cytotoxic machinery. Name one protein that supports the claim.
What is Granzyme B or Perforin?
You are given only “CD3⁺ CD8⁺.” Explain why that isn't enough information to decide whether the cell is resting, activated, proliferating, or cytotoxic.
Because CD3/CD8 establish identity and lineage, but markers of activation, proliferation, and effector function are still needed.